I had a passion to learn and do research in Japan which was grown during my first visit to Japan to attend 6th HOPE meeting with Nobel laureates, Tokyo, 2014. Therefore, I express my deepest appreciation to JICA-Kirin and NFRI, NARO for providing me the opportunity to meet my passion of doing research in Japan. I am very much delighted with the politeness, sincerity, discipline, helpfulness of the Japaness people as well as the culture and the enviornment of Japan. I am very much fond of the charming sceneries around my host institute especially the pattern of housing and the agricultural works on the fields. The NFRI has an excellent research facility as well as environment to conduct high quality research. I have to learn many things here such as how to succeed in research. The learning from NFRI will be of great help to impliment my research in my home institute as well as in my country. I am now doing research on "Transposon mutagenesis of Bacillus spp. to explore the genetic regulation of secondary metabolite production" in Applied Microbiology Unit under the guidance of my adviser, Dr. Keitarou Kimura. This research is very important to understand the genetic regulation of production of metabolite to promote the usage of microbial pesticide in agriculture and food production sector which could in turn keep the environment healthy by subsiding chemical pesticide. My adviser teaches me every thing of molecular biology research. His guidance forces me to succeed in research sector. Everyday, after finishing research work he will say "see you tomorrow" which ispires me to think about the next day’s work plan. Therefore, my hertfelt thanks to my reverend adviser Dr. Keitarou Kimura. All the members of this lab are found very nice and very helpful and I appreciate discipline and hardworkingness of my lab mates. Finally, I am looking forward to achieving a wonderful experience of the advanced research from Applied Microbiology Unit, NFRI by attending JICA-Kirin fellowship program.